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Table 1 The result of the Wight test of blood sera samples after the challenge

From: In vivo immunogenicity assessment and vaccine efficacy evaluation of a chimeric tandem repeat of epitopic region of OMP31 antigen fused to interleukin 2 (IL-2) against Brucella melitensis in BALB/c mice

 

Tteatments

Concentration of sera

OMP31

IL2

3E

OMP-IL2

OMP+IL2

3E-IL2

3E+IL2

OMP-IL2+ IFA

OMP+ IFA

Rev1

LPSa

IFA

PBS

1:10

4+

4+

4+

3+

4+

3+

4+

3+

4+

3+

4+

4+

4+

1:20

4+

4+

4+

2+

3+

2+

4+

2+

4+

2+

4+

4+

4+

1:40

3+

3+

3+

1+

3+

1+

3+

1+

1+

-

4+

4+

4+

1:80

2+

3+

3+

-

1+

-

2+

-

-

-

4+

3+

3+

1:160

-

2+

2+

-

-

-

2+

-

-

-

3+

3+

3+

1:320

-

-

-

-

-

-

-

-

-

-

3+

3+

3+

1:640

-

-

-

-

-

-

-

-

-

-

1+

2+

2+

  1. If all the antigens get agglutinated and the supernatant be clear, the answer is +4
  2. If 75% of antigens get agglutinated and the supernatant be relatively cloudy, the answer is +3
  3. If 50% of antigens get agglutinated and the supernatant be relatively cloudy, the answer is +2
  4. If 25% of antigens get agglutinated and the supernatant be cloudy, the answer is +1
  5. If no sediment is seen and the fluid be completely cloudy, the answer is negative
  6. aThis treatment contains an extract of the periplasmic part of the BL21 (DE3) bacterium which may contains bacterial lipopolysaccharide (LPS)