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Table 1 The result of the Wight test of blood sera samples after the challenge

From: In vivo immunogenicity assessment and vaccine efficacy evaluation of a chimeric tandem repeat of epitopic region of OMP31 antigen fused to interleukin 2 (IL-2) against Brucella melitensis in BALB/c mice

 Tteatments
Concentration of seraOMP31IL23EOMP-IL2OMP+IL23E-IL23E+IL2OMP-IL2+ IFAOMP+ IFARev1LPSaIFAPBS
1:104+4+4+3+4+3+4+3+4+3+4+4+4+
1:204+4+4+2+3+2+4+2+4+2+4+4+4+
1:403+3+3+1+3+1+3+1+1+-4+4+4+
1:802+3+3+-1+-2+---4+3+3+
1:160-2+2+---2+---3+3+3+
1:320----------3+3+3+
1:640----------1+2+2+
  1. If all the antigens get agglutinated and the supernatant be clear, the answer is +4
  2. If 75% of antigens get agglutinated and the supernatant be relatively cloudy, the answer is +3
  3. If 50% of antigens get agglutinated and the supernatant be relatively cloudy, the answer is +2
  4. If 25% of antigens get agglutinated and the supernatant be cloudy, the answer is +1
  5. If no sediment is seen and the fluid be completely cloudy, the answer is negative
  6. aThis treatment contains an extract of the periplasmic part of the BL21 (DE3) bacterium which may contains bacterial lipopolysaccharide (LPS)
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