Fig. 6

Titer of IgG1 and IgG2a isotypes against 3E protein. a titer comparison of IgG1 and IgG2a isotypes sera against 3E protein in 1/50 dilution of sera. Mean comparison of treatments, with significance level of 0.05, were carried out employing Tukey’s test. b Ratio of IgG2a to IgG1 in different treatments. Levels of each antibody were measured at OD 405 nm by an ELISA reader. Each value represents the mean of triplicates ± SD of antibody responses from five samples. The experiment was replicated three times. Different letters indicate significant mean difference between experimental groups (P < .05)