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Fig. 5 | BMC Veterinary Research

Fig. 5

From: In vivo immunogenicity assessment and vaccine efficacy evaluation of a chimeric tandem repeat of epitopic region of OMP31 antigen fused to interleukin 2 (IL-2) against Brucella melitensis in BALB/c mice

Fig. 5

Titer of IgG1 and IgG2a isotypes against OMP31 protein. a the titer comparison of IgG1 and IgG2a isotypes related to 1/50 dilution of sera against OMP31 protein. Mean comparison of treatments, with significance level of 0.05, were carried out using Tukey’s test. b Ratio of IgG2a to IgG1 in different treatments. Levels of each antibody were measured at OD 405 nm by an ELISA reader. Each value represents the mean of triplicates ± SD of antibody responses from five samples. The experiment was replicated three times. Different letters refer to significantly variations between experimental groups (P < .05)

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