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Fig. 1 | BMC Veterinary Research

Fig. 1

From: In vivo immunogenicity assessment and vaccine efficacy evaluation of a chimeric tandem repeat of epitopic region of OMP31 antigen fused to interleukin 2 (IL-2) against Brucella melitensis in BALB/c mice

Fig. 1

Preparation and confirmation of production the recombinant protein. a confirmation of inserting the recombinant gens into pET-22b (+) vector through colony-PCR process using T7 universal primer. b SDS-PAGE analysis which approves purification the recombinant proteins correctly using Ni-NTA column. c Western blot confirmation of recombinant proteins by Anti Poly-Histidine-HRP antibody

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