Fig. 1

Preparation and confirmation of production the recombinant protein. a confirmation of inserting the recombinant gens into pET-22b (+) vector through colony-PCR process using T7 universal primer. b SDS-PAGE analysis which approves purification the recombinant proteins correctly using Ni-NTA column. c Western blot confirmation of recombinant proteins by Anti Poly-Histidine-HRP antibody