Table 1 Validation results of the analytical method developed for adrenal glands. Intraday precision and accuracy were calculated at three concentration levels (low, intermediate, high)
Analyte | Instrumental linearity range (μg L− 1) | R2 | Intraday precision (CV%) | Accuracy (bias %) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
0.5 μg Kg− 1 | 2.0 μg Kg− 1 | 10.0 μg Kg− 1 | 20.0 μg Kg− 1 | 0.5 μg Kg− 1 | 2.0 μg Kg− 1 | 10.0 μg Kg− 1 | 20.0 μg Kg− 1 | |||
6β-hydroxyprednisolone | 0.10–1.0 | 0.9953 | – | 6 | 8 | 8 | – | + 4 | 0 | −7 |
20α-dihydroprednisolone | 0.10–1.0 | 0.9906 | – | 9 | 5 | 12 | – | − 13 | − 5 | + 5 |
20β-dihydroprednisolone | 0.10–1.0 | 0.9919 | – | 11 | 10 | 12 | – | + 4 | + 1 | −1 |
20β-dihydroprednisone | 0.10–1.0 | 0.9900 | – | 8 | 9 | 7 | – | −9 | −6 | + 5 |
Prednisolone | 0.025–0.50 | 0.9988 | 6 | 8 | 7 | – | + 10 | + 6 | + 4 | – |
Prednisone | 0.025–0.50 | 0.9995 | 11 | 5 | 9 | – | + 7 | + 3 | + 1 | – |
Cortisol | 0.50–2.5 | 0.9962 | ||||||||
Cortisone | 0.50–5.0 | 0.9983 | ||||||||