Fig. 5

Phenotype and antibody-dependent cellular cytotoxicity (ADCC) of cultured canine NK cells. a Phenotypic characteristics of expanded non-B, non-T NK lymphocytes. PBMCs (day 0) and expanded NK cells (day 17) were stained with anti-CD3, −CD5, −CD21, −TCRαβ, and -TCRγδ and were analyzed via flow cytometry. Expanded NK cells (CD3⁻CD21⁻CD5⁻TCRαβ⁻TCRγδ⁻) exhibiting over 90% purity after culture were used to evaluate the ADCC effects of such cells in canine tumor cells. b The ADCC ability of expanded NK cells against target tumor cells pretreated with trastuzumab or cetuximab. The 4-h cytotoxicities of NK cells were measured at a 4:1 effector-to-target (E:T) ratio from triplicate reactions and 12 different donors. The cytotoxicity of NK cells against tumor cells pretreated with media alone or human isotype IgG antibody served as controls. The median, first (Q1) and third (Q3) quartiles, and the minimum and maximum are shown. *p < 0.05, **p < 0.01