Fig. 8

Treatment effect on viral RNA synthesis in BVDV-infected cells after 24 h of infection (RT-qPCR). a single compounds or their mixtures present for 2 h before infection (preincubation) or during viral adsorption, b single compounds or their mixtures present only after viral adsorption, c single compounds or their mixtures present both during and after adsorption. Amounts of intracellular BVDV RNA from control (compound-untreated) cells set as 1, results obtained from treated cells expressed as the relative amount of the control virus RNA. Nisin (N) concentrations in μg/mL, lysozyme (Lys) and lactoferrin (LF) concentrations in mg/mL. All data expressed as means ± SD (standard deviation) for n = 3 independent experiments. Asterisks refer to statistically significant differences between control and compound-treated virus at: *p<0.05, ** p<0.01, *** p<0.001