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Table 2 RT-qPCR to determine Getah virus in mosquito samples in northeastern China in 2018

From: First isolation and characterization of Getah virus from cattle in northeastern China

Species No. of mosquitoes No. of pools (100 mosquitoes/pool) No. Positive pools MIR of Mosquitoes (‰)
Culex pseudovishnui 1200 12 2 1.67 (2/1200)
Culex tritaeniorhynchus 7500 75 12 1.60 (12/7500)
Anopheles sinensis 3300 33 4 1.21 (4/3300)
Total 12,000 120 18 1.50 (18/12000)
  1. RT-qPCR, quantitative reverse transcription polymerase chain reaction;
  2. MIR, minimum infection rate. MIR uses the assumption that a positive pool contains only one infected mosquito the minimum infection rate, which is calculated: ([number of positive pools/total specimens tested] × 1000) (https://www.cdc.gov/westnile/resourcepages/mosqSurvSoft.html)