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Table 2 RT-qPCR to determine Getah virus in mosquito samples in northeastern China in 2018

From: First isolation and characterization of Getah virus from cattle in northeastern China

Species

No. of mosquitoes

No. of pools (100 mosquitoes/pool)

No. Positive pools

MIR of Mosquitoes (‰)‡

Culex pseudovishnui

1200

12

2

1.67 (2/1200)

Culex tritaeniorhynchus

7500

75

12

1.60 (12/7500)

Anopheles sinensis

3300

33

4

1.21 (4/3300)

Total

12,000

120

18

1.50 (18/12000)

  1. †RT-qPCR, quantitative reverse transcription polymerase chain reaction;
  2. ‡MIR, minimum infection rate. MIR uses the assumption that a positive pool contains only one infected mosquito the minimum infection rate, which is calculated: ([number of positive pools/total specimens tested] × 1000) (https://www.cdc.gov/westnile/resourcepages/mosqSurvSoft.html)
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BMC Veterinary Research

ISSN: 1746-6148

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