Fig. 1
From: Development of a quick dot blot assay for the titering of bovine ephemeral fever virus
Dot blot assay prepared using antibodies raised against purified BEFV particles. a To immunize rabbits for antiserum production, BEFV particles were isolated by the sucrose gradient centrifugation method and imaged by TEM at 50,000x magnification. b Dot blot assays were set up with raised rabbit anti-BEFV or negative control antiserum as primary antibodies. Two-fold serial dilutions of BEFV test samples, or BHK-21 cell supernatants, were applied for BEFV detection