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Table 2 Combinations of primary and secondary antibodies used on cell cultures and tissue sections

From: Tropism of Newcastle disease virus strains for chicken neurons, astrocytes, oligodendrocytes, and microglia

Primary antibody for cell marker

Secondary (wavelength absorbance in nm)

Primary antibody for NDV infection

Secondary (wavelength absorbance in nm)

GFAPa

Red (594) goat anti-rabbit

NDV-BM

Green (488) goat anti-mouse

TUJ-1a

Green (488) goat anti-mouse

NDV-AP

Red (594) goat anti-rabbit

OLIG-2a

Red (594) goat anti-rabbit

NDV-BM

Green (488) goat anti-mouse

GFAPb

Red (555) goat anti-mouse

NDV NP synthetic peptide

Green (488) goat anti-rabbit

NeuNb

Red (555) goat anti-mouse

NDV-NP synthetic peptide

Green (488) goat anti-rabbit

RCA-1b

Red (555) Biotin binding streptavidin

NDV-NP synthetic peptide

Green (488) goat anti-rabbit

  1. aantibodies used in the DIFA for chicken neural cells (in vitro) infected with LaSota and TxGB NDV strains
  2. bantibodies used in the DIFA for chicken brains (in vivo) infected with SA60, TxGB and Tx450 NDV strains