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Table 1 Primary antibodies used on cell cultures and tissue sections

From: Tropism of Newcastle disease virus strains for chicken neurons, astrocytes, oligodendrocytes, and microglia

Antibody / Cell

Dilution / Isotype

Raised / Clonality / Cell localization

GFAP / Astrocytesa (Abcam #16997)

1:200 / IgG

Rabbit / Polyclonal / Cytoplasmic

GFAP / Astrocytesb (MU020-UC)

1:200 / IgG1

Mouse / Monoclonal / Cytoplasmic

TUJ-1 / Neuronsa (Abcam #14545)

1:1000 / IgG2a

Mouse / Monoclonal / Cytoplasmic

Neu-N / Neuronsb (MAB377)

1:400/ IgG1

Mouse /Monoclonal / Nuclear

OLIG-2 / Oligodendrocytesa

(Genetex #62440)

1:250 / IgG

Rabbit / Monoclonal / Nuclear

Biotinylated Ricinus communis Agglutinin-1 (RCA-1)b

(B-1085)

1:500 / Biotinylated Lectin

Cytoplasmic

Anti-NDV-NPa

1:1000 / IgG

Rabbit / Polyclonal / Cytoplasmic

NDV-BM (Novus Biologicals #NBP2–11633)

1:2000 / RNP*

Mouse / Monoclonal /Cytoplasmic

Anti-NDV-NPb

1:800 / IgG

Rabbit / Polyclonal / Cytoplasmic

  1. afor double immunofluorescent assay (in vitro)
  2. bfor double immunofluorescent assay (in vivo)
  3. *RNP; Ribonucleoprotein