Fig. 3

Representative fields of chicken brains infected with NDV a: infected with NDV SA60, b: infected with NDV TxGB, c: infected with NDV Tx450) and co-labeled for cellular markers. In all sections, NDV immunostaining is green and all sections were counterstained with DAPI (blue) to highlight nuclei. a IFA for Neu-N (pseudo-colored red). Infected neurons show perinuclear and granular immunofluorescence (pseudo-colored green) adjacent to Neu-N positive nuclei. b Fluorescent lectin histochemistry for RCA-I reactivity (pseudo-colored red). Infected microglia demonstrate co-localization of the fluorescent signal (white arrow). c IFA for GFAP (pseudo-colored red). Infected astrocytes demonstrate co-localization of the fluorescent signal (white arrow) in the cytoplasmic projections of GFAP-positive cells