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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Establishment and evaluation of a PRRSV-sensitive porcine endometrial epithelial cell line by transfecting SV40 large T antigen

Fig. 3

Characterization of the PEC cell line. a The comparison of proliferation between the PEC cell line and primary PECs. b Cell cycle comparison between primary PECs and the PEC cell line. (*:p < 0.05) All data were analysed using SPASS software. ANOVA was performed by one-way analysis. Values with P < 0.05 indicated a significant difference between the groups. c Western blot detection of ERɑ and PR. Expression of ERɑ and PR proteins are both shown in primary PECs and the PEC cell line. All data were analysed using SPASS software. ANOVA was performed by one-way analysis. Values with P < 0.05 indicated a significant difference between the groups. (*:p < 0.05) (D) RT-PCR detection of SV40T transcription. SV40T can be detected in the PEC cell line but not in primary PECs. e Karyotype analysis of the PEC cell line shows that the cells display a normal porcine karyotype. f The PEC cell line isolated was determined positive by immunofluorescence testing for cytokeratin CK18, ERɑ and PR, which confirmed its endometrial epithelial origin

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BMC Veterinary Research

ISSN: 1746-6148

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