Fig. 2

Process of SV40T transfection and obtaining a PEC cell line. a Lentiviral particles were packaged and used to infect primary PECs. After selection using puromycin, monoclonal cells were obtained. b Green fluorescence can be seen four days after infection of lentiviral particles. c Cellular morphology of the PEC cell line at 5, 10, 20, 30, 40 and 50 passages. No morphological differences were observed