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Table 5 In vitro and in vivo anthelmintic activity against Haemonchus contortus

From: Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep

Part A: In vitro Concentration (μg/mL)
Egg hatch test ED50 ± SD Methanolic extract Aqueous extract
Mix1 539.2 ± 174.4 940.0 ± 73.0**
Mix2 486.9 ± 228.9 880.0 ± 67.8**
Larval development test LD50 ± SD
Mix1 266.6 ± 107.2 922.7 ± 232.1**
Mix2 826.4 ± 370.5 1256.5 ± 465.0**
Part B: In vivo Numbers of adult worms
Mean number of adult worms ± SD Infected animals Infected animals with Mix1 Infected animals with Mix2
  1523 ± 187 1113 ± 274* 1327 ± 351
  1. In vitro activity: data are means ± SDs of three independent assays with duplicate samples. Ovicidal and larvicidal activities are expressed as the concentration (μg/mL) of a median lethal dose (ED50 and LD50, respectively; the concentration of a methanolic or aqueous extract that prevents 50% of the eggs from hatching or larvae from developing to the infective L3 stage) using the egg hatch test or larval development test
  2. In vivo activity: *Reduction of adult worms relative to infected animals 70 days after infection (P < 0.05)
  3. **Means within a row are significantly different at P < 0.01