Fig. 1
From: Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain
Expression and purification of rMmPrdx6. a Expression of the recombinant MmPrdx6 protein. M: Protein marker (Sangon, China); Lane 1: Total protein from induced E. coli BL21(DE3) cells harbouring pET-28a-Prdx6; Lane 2: Inclusion bodies of the induced E. coli BL21(DE3) cells harbouring pET-28a-Prdx6; Lane 3: Bacterial supernatants of the induced E. coli BL21(DE3) cells harbouring pET-28a-Prdx6; Lane 4: Total protein from uninduced E. coli BL21(DE3) cells harbouring pET-28a-Prdx6. b Purification of recombinant MmPrdx6 with a Ni-NTA column and visualization by SDS-PAGE. M: Protein marker; Lane 1: Bacterial supernatant of induced E. coli BL21(DE3) harbouring pET-28a-Prdx6; Lane 2: Purified rMmPrdx6 (with His-tag); c Identification of recombinant MmPrdx6 by western blotting. M: protein marker (Sangon, China); Lane 1: Purified rMmPrdx6