Fig. 4From: The effect of four different freezing conditions and time in frozen storage on the concentration of commonly measured growth factors and enzymes in equine platelet-rich plasma over six monthsMatrix metalloproteinase-9 (MMP-9) fold change in platelet-rich plasma (PRP) at 1, 3, and 6 months of storage in -20 °C automatic defrost freezer (− 20A), −20 °C manual defrost freezer (−20 M), − 80 °C manual defrost freezer (− 80 M), and liquid nitrogen (LN) compared to baseline fresh activated PRP at time 0 (PRP0) and snap-frozen PRP at time 0 (LN0). All samples were activated with bovine thrombin and calcium chloride prior to growth factor quantification at each time point. Bars represent mean (n = 6) ± standard error. Differing letters indicate a significant difference between groups within storage method, using a repeated measures ANOVA with a Tukey post-hoc test, p < 0.05Back to article page