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Table 3 Primers used for the three different PCRs

From: Comparison of three different PCR protocols for the detection of ferlaviruses

RT-PCR Primer name Primer sequence Expected size of final PCR product (with primers) Reference
I F5-outer 5′-GCAGAGATTTTCTCTTTCTT-3’ 566 bp [17]
R6-outer 5′-AGCTCTCATTTTGTATGTCAT-3’
F7-inner 5′-TAGAGGCTGTTACTGCTGC-3’
R8-inner 5′-CATCTTGGCAAATAATCAGCC-3’
II RES-MOR-HEN-F1 5′-TCITTCTTTAGAACITTYGGNCAYCC-3’ 500 bp [26]
RES-MOR-HEN-F2 5′-GCCATATTTTGTGGAATAATHATHAAYGG-3’
RES-MOR-HEN-R 5′-CTCATTTTGTAIGTCATYTTNGCRAA-3’
III S2 5′-GTTATGGCAAATCATGCTGCGATACCTTA-3’ 149 bp [27]
AS2 5′-CTGATGGGAGATAATGCCTTGTCCTTCAT-3’