Table 3 Primers used for the three different PCRs
From: Comparison of three different PCR protocols for the detection of ferlaviruses
RT-PCR | Primer name | Primer sequence | Expected size of final PCR product (with primers) | Reference |
|---|---|---|---|---|
I | F5-outer | 5′-GCAGAGATTTTCTCTTTCTT-3’ | 566 bp | [17] |
R6-outer | 5′-AGCTCTCATTTTGTATGTCAT-3’ | |||
F7-inner | 5′-TAGAGGCTGTTACTGCTGC-3’ | |||
R8-inner | 5′-CATCTTGGCAAATAATCAGCC-3’ | |||
II | RES-MOR-HEN-F1 | 5′-TCITTCTTTAGAACITTYGGNCAYCC-3’ | 500 bp | [26] |
RES-MOR-HEN-F2 | 5′-GCCATATTTTGTGGAATAATHATHAAYGG-3’ | |||
RES-MOR-HEN-R | 5′-CTCATTTTGTAIGTCATYTTNGCRAA-3’ | |||
III | S2 | 5′-GTTATGGCAAATCATGCTGCGATACCTTA-3’ | 149 bp | [27] |
AS2 | 5′-CTGATGGGAGATAATGCCTTGTCCTTCAT-3’ |