Table 2 Tissue samples from experimentally infected corn snakes tested using three different PCR protocols (numbering the same as in Pees et al. [21])
From: Comparison of three different PCR protocols for the detection of ferlaviruses
No. | Sample material | PCR I [17] | PCR II [26] | PCR III [27] |
|---|---|---|---|---|
Genogroup A | ||||
1 | lung | – | – | + |
brain | – | – | – | |
2 | lung | + | (((+))) | + |
brain | – | – | – | |
3 | lung | + | – | + |
brain | – | – | – | |
4 | lung | – | – | + |
brain | – | – | – | |
5 | lung | + | – | + |
brain | + | – | (+) | |
6 | lung | + | – | + |
brain | (+) | – | ((+)) | |
7 | lung | – | – | – |
brain | – | – | – | |
8 | lung | + | – | + |
brain | + | – | + | |
9 | lung | – | – | + |
brain | + | – | + | |
10 | lung | – | – | – |
brain | – | – | – | |
11 | lung | + | – | + |
brain | + | – | + | |
12 | lung | – | – | – |
brain | – | – | – | |
Total positive | n = 24 | 11 | 1 | 14 |
Genogroup B | ||||
1 | lung | – | ((+)) | – |
brain | – | – | – | |
2 | lung | + | ((+)) | + |
brain | – | – | – | |
3 | lung | (+) | – | + |
brain | – | – | – | |
4 | lung | + | (((+))) | + |
brain | – | – | (+) | |
5 | lung | – | ((+)) | + |
brain | – | – | – | |
6 | lung | (+) | (((+))) | + |
brain | – | – | – | |
7 | lung | + | (((+))) | + |
brain | + | – | + | |
8 | lung | (+) | – | + |
brain | + | – | (+) | |
9 | lung | + | – | + |
brain | + | – | + | |
10 | lung | + | – | + |
brain | + | – | + | |
11 | lung | – | ((+)) | + |
brain | – | – | – | |
12 | lung | + | ((+)) | + |
brain | + | – | (+) | |
Total positive | n = 24 | 14 | 8 | 17 |
Genogroup C | ||||
1 | lung | + | – | + |
2 | lung | + | (+) | + |
brain | + | – | + | |
3 | lung | + | – | ((+)) |
4 | lung | + | – | – |
brain | + | – | + | |
5 | lung | + | + | + |
6 | lung | + | – | (+) |
7 | lung | + | (+) | + |
brain | – | – | – | |
8 | lung | – | – | – |
9 | lung | + | – | – |
10 | lung | + | – | + |
11 | lung | + | + | + |
12 | lung | – | – | – |
Total positive | n = 15 | 12 | 4 | 10 |