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Table 2 Tissue samples from experimentally infected corn snakes tested using three different PCR protocols (numbering the same as in Pees et al. [21])

From: Comparison of three different PCR protocols for the detection of ferlaviruses

No. Sample material PCR I [17] PCR II [26] PCR III [27]
Genogroup A
 1 lung +
brain
 2 lung + (((+))) +
brain
 3 lung + +
brain
 4 lung +
brain
 5 lung + +
brain + (+)
 6 lung + +
brain (+) ((+))
 7 lung
brain
 8 lung + +
brain + +
 9 lung +
brain + +
 10 lung
brain
 11 lung + +
brain + +
 12 lung
brain
Total positive n = 24 11 1 14
Genogroup B
 1 lung ((+))
brain
 2 lung + ((+)) +
brain
 3 lung (+) +
brain
 4 lung + (((+))) +
brain (+)
 5 lung ((+)) +
brain
 6 lung (+) (((+))) +
brain
 7 lung + (((+))) +
brain + +
 8 lung (+) +
brain + (+)
 9 lung + +
brain + +
 10 lung + +
brain + +
 11 lung ((+)) +
brain
 12 lung + ((+)) +
brain + (+)
Total positive n = 24 14 8 17
Genogroup C
 1 lung + +
 2 lung + (+) +
brain + +
 3 lung + ((+))
 4 lung +
brain + +
 5 lung + + +
 6 lung + (+)
 7 lung + (+) +
brain
 8 lung
 9 lung +
 10 lung + +
 11 lung + + +
 12 lung
Total positive n = 15 12 4 10
  1. -: negative, +: positive amplification product with a strong band, (+): positive amplification product with a weaker band, ((+)): positive amplification product with a weak band, (((+))): positive amplification product with a very weak band. Examples for different bank strengths are shown in Additional file 1: Figure S1