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Table 1 Dilution series of the cell culture isolates tested using three different PCR protocols

From: Comparison of three different PCR protocols for the detection of ferlaviruses

Concentration of RNA from cell culture isolates PCR I [17] PCR II [26] PCR III [27]
Xeno-USA99
 5 ng/μL + (+) +
 5 × 10− 1 ng/μL + +
 5 × 10− 2 ng/μL ((+))
 5 × 10− 3 ng/μL
 5 × 10− 4 ng/μL
Crot-GER03
 25 ng/μL + +
 6 ng/μL + +
 5 × 10− 1 ng/μL + +
 5 × 10− 2 ng/μL + +
 5 × 10− 3 ng/μL + (+)
 5 × 10− 4 ng/μL
PanGut-GER09
 5 ng/μL + +
 5 × 10− 1 ng/μL + (+)
 5 × 10− 2 ng/μL
 5 × 10− 3 ng/μL
 5 × 10− 4 ng/μL
Ther-GER99
 7 ng/μL ++ + +
 5 × 10−1 ng/μL + +
 5 × 10− 2 ng/μL (+)
 5 × 10− 3 ng/μL
 5 × 10− 4 ng/μL
  1. -: negative, +: positive amplification product with a strong band, (+): positive amplification product with a weaker band, ((+)): positive amplification product with a weak band, ++: positive amplification product of the expected size, with an unspecific lower band, −+: negative PCR result, with an unspecific lower band