Table 1 Dilution series of the cell culture isolates tested using three different PCR protocols
From: Comparison of three different PCR protocols for the detection of ferlaviruses
Concentration of RNA from cell culture isolates | PCR I [17] | PCR II [26] | PCR III [27] |
|---|---|---|---|
Xeno-USA99 | |||
5 ng/μL | + | (+) | + |
5 × 10− 1 ng/μL | + | – | + |
5 × 10− 2 ng/μL | – | – | ((+)) |
5 × 10− 3 ng/μL | – | – | – |
5 × 10− 4 ng/μL | – | – | – |
Crot-GER03 | |||
25 ng/μL | + | – | + |
6 ng/μL | + | – | + |
5 × 10− 1 ng/μL | + | – | + |
5 × 10− 2 ng/μL | + | – | + |
5 × 10− 3 ng/μL | + | – | (+) |
5 × 10− 4 ng/μL | – | – | – |
PanGut-GER09 | |||
5 ng/μL | + | – | + |
5 × 10− 1 ng/μL | + | – | (+) |
5 × 10− 2 ng/μL | – | – | – |
5 × 10− 3 ng/μL | – | – | – |
5 × 10− 4 ng/μL | – | – | – |
Ther-GER99 | |||
7 ng/μL | ++ | + | + |
5 × 10−1 ng/μL | −+ | – | + |
5 × 10− 2 ng/μL | – | – | (+) |
5 × 10− 3 ng/μL | – | – | – |
5 × 10− 4 ng/μL | – | – | – |