Fig. 1From: The development of an indirect ELISA for the detection of goose parvovirus antibodies using specific VP3 subunits as the coating antigenExpression and purification of VP3ep6 and VP3ep4–6 analyzed by SDS–PAGE. Lane 1 – molecular mass marker (ThermoFisher Scientific), lane 2 – soluble protein extracts from E. coli strain Rossetta (DE3) pLysS + pUET1, lane 3 – soluble protein extracts from E. coli strain Rossetta (DE3) pLysS + pUET1-VP3ep6, lane 4 – purified VP3ep6, lane 5 – soluble protein extracts from E. coli strain Rossetta (DE3) pLysS + pUET1-VP3ep4–6, lane 6 – purified VP3ep4–6Back to article page