Table 2 Sequence, source, amplicon size of primers used for PCR analysis. Primers are grouped in different categories
From: Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells
Primers | References | Sequences (5′ → 3′) | bp |
|---|---|---|---|
Mesenchymal markers | |||
 CD90 | [52] | FW: TGCGAACTCCGCCTCTCT | 93 |
| Â | RW: GCTTATGCCCTCGCACTTG | ||
 CD73 | [52] | FW: GGGATTGTTGGATACACTTCAAAAG RW: GCTGCAACGCAGTGATTTCA | 90 |
Hematopoietic markers | |||
 CD34 | [52] | FW: CACTAAACCCTCTACATCATTTTCTCCTA | 101 |
| Â | RW: GGCAGATACCTTGAGTCAATTTCA | ||
 CD45 | [52] | FW: TGATTCCCAGAAATGACCATGTA | 101 |
| Â | RW: ACATTTTGGGCTTGTCCTGTAAC | ||
Major histocompatibility complex markers | |||
 MHC1 | [53] | FW: GGAGAGGAGCAGAGATACA | 218 |
| Â | RW: CTGTCACTGTTTGCAGTCT | ||
 MHC2 | [53] | FW: TCTACACCTGCCAAGTG | 178 |
| Â | RW: CCACCATGCCCTTTCTG | ||
Pro- and anti-inflammatory cytokines | |||
 TNFα | [54] | FW: GCTCCAGACGGTGCTTGTG | 95 |
| Â | RW: GCCGATCACCCCAAAGTG | ||
 IL8 | [54] | FW: CGGTGCCAGTGCATCAAG | 81 |
| Â | RW: TGGCCCACTCTCAATCACTCT | ||
 INFγ | [55] | FW: GTGTGCGATTTTGGGTTCTTCTA | 235 |
| Â | RW: TTGAATGACCTGGTTATCT | ||
 IL4 | [55] | FW: CAACTTCATCCAGGGATGCAA | 107 |
| Â | RW: CAGTCAGCTCCATGCACGAAT | ||
 ILβ1 | [55] | FW: GAGGCAGCCATGGCAGCAGTA | 257 |
| Â | RW: TGTGAGCAGGGAACGGGTATCTT | ||
 IL6 | [56] | FW: AAACCACCTCAAATGGACCACTA | 91 |
| Â | RW: TTTTTCAGGGCAGAGATTTTGC | ||
Pluripotency markers | |||
 OCT4 | [36] | FW: TCCCAGGACATCAAAGCTCTGCAGA | 679 |
| Â | RW: TCAGTTTGAATGCATGGGAGAAGCCCAGA | ||
 NANOG | [36] | FW: GACAGCCCCGATTCATCCACCAG | 492 |
| Â | RW: GCACCAGGTCTGACTGTTCCAGG | ||
 SOX2 | [36] | FW: GGCGGCAACCAGAAGAACAG | 663 |
| Â | RW: AGAAGAGGTAACCACGGGGG | ||
Housekeeping | |||
 GAPDH | [36] | FW: GTCCATGCCATCACTGCCAC | 262 |
| Â | RW: CCTGCTTCACCACCTTCTTG | ||