Fig. 8

TEM analysis of equine adult mesenchymal stem cells (MSCs). Bone marrow MSCs (a) Cells show a fibroblast like morphology. Nucleus (N) and dark and dense nucleoli (n) are well detected (bar: 20 μm); b A well-developed Golgi complex surrounded by several vesicles and multivesicular bodies (white square; bar: 500 nm) are observed in the cytoplasm (bar: 1 μm); c Extended RER (rer), characterized by dilated cisternae, lacking of ribosomes, is shown in the cytoplasm (bar: 500 nm); d Long and narrow mitochondria (m), with dense matrix and thin cristae, are observed in the cytoplasm. Extracellular vesicles (black arrow) and exosomes (black arrowhead) are detected in the extracellular environment (bar: 1 μm). Adipose tissue MSCs (e) low magnification image showing a cluster of MSCs with a spindle morphology, nucleus (N) and dark and dense nucleoli (n) (bar: 10 μm); f Golgi apparatus (black arrow) and mitochondria (m) were observed in the cytoplasm (bar: 1 μm); g detail of the cytoplasm showing an extended RER (rer) characterized by dilated cisternae almost empty of ribosomes (bar: 1 μm); (H) (bar: 500 nm); h Aggregated extracellular vesicles (black arrow) are localized nearby cell membrane (bar: 500 nm)