Skip to main content

Table 2 Primers used in virotyping PCR reactions, with their sequence, size of ampliconsand biological function of targeted genes

From: Comparison of the phylogenetic analysis of PFGE profiles and the characteristic of virulence genes in clinical and reptile associated Salmonella strains

Gene target Primer sequence Amplicon size (bp) Function of gene
invA F-CTGGCGGTGGGTTTTGTTGTCTTCTCTATT 1070 Host recognition/invasion
R-AGTTTCTCCCCCTCTTCATGCGTTACCC
orgA F-TTTTTGGCAATGCATCAGGGAACA 255 Host recognition/invasion
R-GGCGAAAGCGGGGACGGTATT
prgH F-GCCCGAGCAGCCTGAGAAGTTAGAAA 756 Host recognition/invasion
R-TGAAATGAGCGCCCCTTGAGCCAGTC
spaN F-AAAAGCCGTGGAATCCGTTAGTGAAGT 504 Entry into nonphagocytic cells, killing of macrophages
R-CAGCGCTGGGGATTACCGTTTTG
tolC F-TACCCAGGCGCAAAAAGAGGCTATC 161 Host recognition/invasion
R-CCGCGTTATCCAGGTTGTTGC
sipB F-GGACGCCGCCCGGGAAAAACTCTC 875 Entry into nonphagocytic cells, killing of macrophages
R-ACACTCCCGTCGCCGCCTTCACAA
sitC F-CAGTATATGCTCAACGCGATGTGGGTCTCC 768 Iron acquisition
R-CGGGGCGAAAATAAAGGCTGTGATGAAC
pagC F-CGCCTTTTCCGTGGGGTATGC 454 Survival within macrophage
R-GAAGCCGTTTATTTTTGTAGAGGAGATGTT
msgA F-GCCAGGCGCACGCGAAATCATCC 189 Survival within macrophage
R-GCGACCAGCCACATATCAGCCTCTTCAAAC
spiA F-CCAGGGGTCGTTAGTGTATTGCGTGAGATG 550 Survival within macrophage
R-CGCGTAACAAAGAACCCGTAGTGATGGATT
iroN F-ACTGGCACGGCTCGCTGTCGCTCTAT 1205 Iron acquisition
R-CGCTTTACCGCCGTTCTGCCACTGC
sopB F-GGACCGGCCAGCAACAAAACAAGAAGAAG 220 Host recognition/invasion
R-TAGTGATGCCCGTTATGCGTGAGTGTATT
lpfC F-GCCCCGCCTGAAGCCTGTGTTGC 641 Host recognition/invasion
R-AGGTCGCCGCTGTTTGAGGTTGGATA
cdtB F-ACAACTGTCGCATCTCGCCCCGTCATT 268 Host recognition/invasion
R-CAATTTGCGTGGGTTCTGTAGGTGCGAGT
sifA F-TTTGCCGAACGCGCCCCCACACG 449 Filamentous structure formation
R-GTTGCCTTTTCTTGCGCTTTCCACCCATCT
pefA F-GCGCCGCTCAGCCGAACCAG 157 Host recognition/invasion
R-GCAGCAGAAGCCCAGGAAACAGTG
spvB F-CTATCAGCCCCGCACGGAGAGCAGTTTTTA 717 Growth within host
R-GGAGGAGGCGGTGGCGGTGGCATCATA