Fig. 3

Experimental design. The diagram illustrates on a timeline the hormone or beta-lactam antibiotic exposure of AH1 and LE cells, infection and samples collection at different times. Cells were pre-exposed to progesterone (P4), estradiol (E2) or penicillin G (Pen G) added in incubation medium for 24 h. At this time, the cells were infected by the AB7 strain of C. abortus and, two hours later, they were again exposed to P4, E2 or Pen G in incubation medium, and incubated until 48 and 72 hpi, when samples were collected for genomic studies. In addition, samples were collected at 72 hpi for morphological studies using transmission electron microscopy (TEM)