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Table 4 Forward and reverse sequence, amplicon size and melt temperature for the high-resolution melt curve analysis distinguishing between blaCTX-M-1 or blaCTX-M-15 within CTX-M group 1

From: Emergence of carriage of CTX-M-15 in faecal Escherichia coli in horses at an equine hospital in the UK; increasing prevalence over a decade (2008–2017)

Primer

Sequence 5 > 3

Amplicon size

Melt Tm

CTXMG1 F

TGTTGTTAGGAAGTGTGCCG

213 bp

84.75 C (CTX-M-1)

CTXMG1 R

CGCTTTCACTTTTCTTCAGCAC

85.25 (CTX-M-15)

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BMC Veterinary Research

ISSN: 1746-6148

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