Fig. 3

Effects of PRP on cell viability of K9BMMSC cells assessed by MTS assay. K9BMMSC cells were treated with increased concentration of PRP (0, 20, 40, 50, 60, 80, 100%) in DMEM/F12 complete media (v/v) for 72 h. Cell viability was determined by MTS assay. PRP at 40% concentration increased cell viability in all tested K9BMMSCs (a-f). Values represented here are mean ± S.E. of four replicates of PRP treated cells normalized to cells cultured in complete DMEM/F12 media only. Paired Student’s t-test was performed to compare differences in cell viability of PRP-treated K9BMMSCs to untreated cells. Significance was determined at ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001. K9BMMSC: canine bone marrow-derived multipotent mesenchymal stromal cells; PRP: platelet-rich plasma