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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro

Fig. 1

Isolation of MSCs from bone marrow and extraction of PRP from whole blood. a Bone marrow was harvested under sedation from the proximal humerus of client-owned dogs with osteoarthritis by a board–certified orthopedic veterinarian. b K9BMMSCs were isolated from the layer of cells (arrow) separated using LSM after centrifugation of bone marrow samples. c K9BMMSC#4 cells cultured in vitro in DMEM/F12 medium 7 days after seeding. d PRP was separated from whole blood using the Dr.PRP PRP kit. The separated layers of plasma (top chamber), PRP (center) and blood cells components (bottom chamber) in a Dr.PRP closed system tube after centrifugation. K9BMMSC: canine bone marrow-derived multipotent mesenchymal stromal cells; PRP: Platelet-rich plasma

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