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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Canine platelets express functional Toll-like receptor-4: lipopolysaccharide-triggered platelet activation is dependent on adenosine diphosphate and thromboxane A2 in dogs

Fig. 4

ADP-priming augments LPS-mediated alpha-granule secretion in canine platelets. Platelet alpha-granule secretion was assessed by surface P-selectin (CD62P) measured as percent (%) positive or mean fluorescence intensity (MFI) fold change on isolated platelets from 10 dogs using flow cytometry. Thrombin-stimulated platelets served as positive control. a Platelets were treated with 0, 1, 5, or 10 μg/ml LPS. Only platelets treated with 5 μg/mL LPS had significant increase in CD62P+ platelets compared to unstimulated platelets. b,c Platelets were primed with 10 μM ADP prior to stimulation with 0, 1, 5 or 10 μg/ml LPS. LPS stimulation with 1 μg/mL LPS in ADP-primed platelets significantly elevated the percentage of CD62P+ platelets b but did not increase MFI fold change c compared to ADP-primed platelets without LPS. LPS at 5 μg/mL significantly increase CD62P MFI fold change in ADP-primed platelets relative to those without LPS. ADP priming increased platelet response to LPS at 1 (b), 5 μg/mL (b,c) and 10 μg/mL (c). First and third quartiles were represented by the lower and upper boundaries, respectively. + and the line within the box represents the mean and median, respectively. Whiskers represent the range of data. * p < 0.05, # All treatments were significantly different (p < 0.05) from positive control

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