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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Two novel monoclonal antibodies against fiber-1 protein of FAdV-4 and their application in detection of FAdV-4/10

Fig. 4

A novel mAbs based sandwich ELISA for efficient detection of FAdV-4/10 a SDS-PAGE assay for the purified mAb 3B5 and 6H9. Lane 1 and lane 2: the purified mAb 3B5 and 6H9 respectively; Lane3 and lane 4: the unpurified mAb 3B5 and 6H9 respectively; b Specificity assay for the sandwich ELISA using different viruses including FAdV-1, FAdV-4, FAdV-5, FAdV-6, FAdV-7, FAdV-8, FAdV-9, FAdV-10, MDV, AIV, ALV, REV, IBV and CAV; c Sensitivity assay for the sandwich ELISA using different doses of different FAdV-4 isolates; d Detection of FAdV-4 in 32 clinical liver samples using the sandwich ELISA. Three liver samples from the SPF chickens were used as negative control and three liver samples from the chickens experimentally infected with FAdV-4 were used as positive control

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