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Table 6 The diagnostic sensitivity, and diagnostic specificity of conventional PCR and oligonucleotide microarray

From: Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases

Target

Sensitivity a(TP/(TP + FN))

Specificity b (TN/(TN + FP))

conventional PCR

oligonucleotide microarray

conventional PCR

oligonucleotide microarray

Total

94.33% (50/53)

100%(53/53)

100%(40/40)

100%(40/40)

AIV

90.32%(28/31)

100%(31/31)

100%(62/62)

100%(62/62)

H5

88.89%(16/18)

100%(18/18)

100%(75/75)

100%(75/75)

H7

66.67% (6/9)

100%(9/9)

100%(84/84)

100%(84/84)

H9

60% (3/5)

100%(5/5)

100%(88/88)

100%(88/88)

IBV

100% (0/0)

100%(0/0)

100%(93/93)

100%(93/93)

NDV

100% (22/22)

100%(22/22)

100%(71/71)

100%(71/71)

  1. Virus isolation was as the gold standard, the diagnostic sensitivity, and diagnostic specificity of conventional PCR and oligonucleotide microarray for detecting viral were identified in 93 field samples. a TP, true positive; FN, false negative; Sensitivity = TP/(TP + FN) × 100%. b TN, true negative; FP, false positive; Specificity = TN/(TN + FP) × 100%
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BMC Veterinary Research

ISSN: 1746-6148

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