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Table 6 The diagnostic sensitivity, and diagnostic specificity of conventional PCR and oligonucleotide microarray

From: Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases

Target Sensitivity a(TP/(TP + FN)) Specificity b (TN/(TN + FP))
conventional PCR oligonucleotide microarray conventional PCR oligonucleotide microarray
Total 94.33% (50/53) 100%(53/53) 100%(40/40) 100%(40/40)
AIV 90.32%(28/31) 100%(31/31) 100%(62/62) 100%(62/62)
H5 88.89%(16/18) 100%(18/18) 100%(75/75) 100%(75/75)
H7 66.67% (6/9) 100%(9/9) 100%(84/84) 100%(84/84)
H9 60% (3/5) 100%(5/5) 100%(88/88) 100%(88/88)
IBV 100% (0/0) 100%(0/0) 100%(93/93) 100%(93/93)
NDV 100% (22/22) 100%(22/22) 100%(71/71) 100%(71/71)
  1. Virus isolation was as the gold standard, the diagnostic sensitivity, and diagnostic specificity of conventional PCR and oligonucleotide microarray for detecting viral were identified in 93 field samples. a TP, true positive; FN, false negative; Sensitivity = TP/(TP + FN) × 100%. b TN, true negative; FP, false positive; Specificity = TN/(TN + FP) × 100%