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Table 5 Virus detection in field samples by conventional PCR, oligonucleotide microarray and virus isolation

From: Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases

Virus Conventional PCR Oligonucleotide microarray Virus isolationa
Positive Positive Positiveb
Influenza A 28/93 (30.11%) 31/93 (33.33%) 31/93 (33.33%)
H5 16/93 (17.20%) 18/93 (19.35%) 18/93 (19.35%)
H7 6/93 (6.45%) 7/93 (7.53%) 9/93 (9.68%)
H9 3/93 (3.23%) 3/93 (3.23%) 5/93 (5.38%)
H5, H7 0/93 (0%) 0/93 (0%) 0/93 (0%)
H7, H9 0/93 (0%) 2/93 (2.15%) 0/93 (0%)
H5, H9 0/93 (0%) 0/93 (0%) 0/93 (0%)
H5, H7, H9 0/93 (0%) 0/93 (0%) 0/93 (0%)
IBV 0/93 (0%) 0/93 (0%) 0/93 (0%)
NDV 22/93 (23.66%) 22/93 (23.66%) 22/93 (23.66%)
Positive 50/93 (53.76%) 53/93 (56.99%) 53/93 (56.99%)
  1. aVirus isolation was performed on specific-pathogen-free egg embryo
  2. bThe type and subtype were identified by reverse transcription and conventional PCR assay, respectively