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Table 5 Virus detection in field samples by conventional PCR, oligonucleotide microarray and virus isolation

From: Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases

Virus

Conventional PCR

Oligonucleotide microarray

Virus isolationa

Positive

Positive

Positiveb

Influenza A

28/93 (30.11%)

31/93 (33.33%)

31/93 (33.33%)

H5

16/93 (17.20%)

18/93 (19.35%)

18/93 (19.35%)

H7

6/93 (6.45%)

7/93 (7.53%)

9/93 (9.68%)

H9

3/93 (3.23%)

3/93 (3.23%)

5/93 (5.38%)

H5, H7

0/93 (0%)

0/93 (0%)

0/93 (0%)

H7, H9

0/93 (0%)

2/93 (2.15%)

0/93 (0%)

H5, H9

0/93 (0%)

0/93 (0%)

0/93 (0%)

H5, H7, H9

0/93 (0%)

0/93 (0%)

0/93 (0%)

IBV

0/93 (0%)

0/93 (0%)

0/93 (0%)

NDV

22/93 (23.66%)

22/93 (23.66%)

22/93 (23.66%)

Positive

50/93 (53.76%)

53/93 (56.99%)

53/93 (56.99%)

  1. aVirus isolation was performed on specific-pathogen-free egg embryo
  2. bThe type and subtype were identified by reverse transcription and conventional PCR assay, respectively