From: A single nucleotide variant in the promoter region of the CCR5 gene increases susceptibility to arthritis encephalitis virus in goats
Buffer 10X
2.5 μl
Mg 2+ (2.5 mM)
DNPTs (0.2 mM)
1.0 μl
Primer ExIF (300 nM)
0.5 μl
Primer ExIIR (300 nM)
GC solution
5 μl
Taq Roche(1.5 U)
0.2 μl
Water
11.8 μl
c-DNA (dil. 1/5)
1 μl