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Table 4 Reagents used for PCR for defining Caprine CCR5 structure

From: A single nucleotide variant in the promoter region of the CCR5 gene increases susceptibility to arthritis encephalitis virus in goats

Buffer 10X

2.5 μl

Mg 2+ (2.5 mM)

2.5 μl

DNPTs (0.2 mM)

1.0 μl

Primer ExIF (300 nM)

0.5 μl

Primer ExIIR (300 nM)

0.5 μl

GC solution

5 μl

Taq Roche(1.5 U)

0.2 μl

Water

11.8 μl

c-DNA (dil. 1/5)

1 μl