Table 6 Selected genes and primers used for gene expression analysis of intestinal tissue
Gene name | Entrez gene ID | Primer sequence | Tm | Product size (bp) | Gene Function |
|---|---|---|---|---|---|
LYN | 100,302,103 | 5′-ACGGAGAGTGGTGGAAAGC-3′ 5′-GTGCACGGGGTCATAGTCT-3’ | 59.6 59.1 | 482 | B cell signaling + activation |
GRB2 | 101,109,893 | 5’-ACTGCTGCTCCTGTTCTTCC-3′ 5′-AAACGCAGAACACAGAAGCG-3’ | 60.0 59.7 | 442 | B cell signal transduction + communication |
EGR1 | 443,547 | 5’-CCCCGACTATCTGTTTCCACA-3′ 5′-ATGCGGCTGGGTTTGATGA-3’ | 59.5 60.0 | 340 | B cell maturation + activating factor signaling |
JUN | 443,219 | 5’-CAAGTGCCGGAAAAGGAAGC-3′ 5′-ACAGTCTCGCCTCAAAACGT-3’ | 59.5 59.8 | 314 | Transcription regulator |
NFIL3 | 100,217,409 | 5’-CACTGTGAGCGCCTTTGTG-3′ 5′-GGGCCCTCCTGTGAATGTT-3’ | 59.7 59.6 | 262 | B Cell survival |
LCK | 100,216,439 | 5’-CCCAGCTTCTCCACTGCAA-3′ 5′-ACGGAGCTGTTCACCCTTC-3’ | 59.9 59.6 | 270 | B cell activation |
CD81 | 100,147,790 | 5’-CTGGGCACGTTCTTCACCT-3′ 5′-GCTGCAAGGCCTGGTCATA-3’ | 59.3 60.0 | 479 | B cell development, activation, growth and motility |
CD84 | 101,102,779 | 5’-CGTGGAACCCTGTCAGCAA-3′ 5′-AGCACAGAAAGCACAGCCA-3’ | 60.2 60.2 | 412 | Found on memory B cells |
CD40L | 767,628 | 5’-AGCTGGCCGTGAAAAGACA-3′ 5′-AACACCGAAGCACCCGATT-3’ | 59.9 59.9 | 489 | Initiation and maintenance of humoral immune response |
BAFF (TNFSF13B) | 101,104,901 | 5’-TTGCAGACAGTGACACGCC-3′ 5′-AGGTGTCCCATGGCAAAGG-3’ | 60.9 59.9 | 426 | B Cell survival |
MIF | 780,466 | 5’-GCAAGCCGGCACAGTACAT-3′ 5′-ATGTAGATCCTGTCCGGGCT-3’ | 61.0 60.1 | 305 | Survival of mature B cells + NF-kB signalling cascade |
Reference gene | |||||
Ovine GAPDH | 443,005 | 5’-AGAAACCTGCCAAGTATGATG-3′ 5′-CCTAGAATGCCCTTGAGAGG-3’ | 60.5 62.6 | 76 |  |