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Table 6 Selected genes and primers used for gene expression analysis of intestinal tissue

From: The humoral immune response is essential for successful vaccine protection against paratuberculosis in sheep

Gene name Entrez gene ID Primer sequence Tm Product size (bp) Gene Function
LYN 100,302,103 5′-ACGGAGAGTGGTGGAAAGC-3′
5′-GTGCACGGGGTCATAGTCT-3’
59.6
59.1
482 B cell signaling + activation
GRB2 101,109,893 5’-ACTGCTGCTCCTGTTCTTCC-3′
5′-AAACGCAGAACACAGAAGCG-3’
60.0
59.7
442 B cell signal transduction + communication
EGR1 443,547 5’-CCCCGACTATCTGTTTCCACA-3′
5′-ATGCGGCTGGGTTTGATGA-3’
59.5
60.0
340 B cell maturation + activating factor signaling
JUN 443,219 5’-CAAGTGCCGGAAAAGGAAGC-3′
5′-ACAGTCTCGCCTCAAAACGT-3’
59.5
59.8
314 Transcription regulator
NFIL3 100,217,409 5’-CACTGTGAGCGCCTTTGTG-3′
5′-GGGCCCTCCTGTGAATGTT-3’
59.7
59.6
262 B Cell survival
LCK 100,216,439 5’-CCCAGCTTCTCCACTGCAA-3′
5′-ACGGAGCTGTTCACCCTTC-3’
59.9
59.6
270 B cell activation
CD81 100,147,790 5’-CTGGGCACGTTCTTCACCT-3′
5′-GCTGCAAGGCCTGGTCATA-3’
59.3
60.0
479 B cell development, activation, growth and motility
CD84 101,102,779 5’-CGTGGAACCCTGTCAGCAA-3′
5′-AGCACAGAAAGCACAGCCA-3’
60.2
60.2
412 Found on memory B cells
CD40L 767,628 5’-AGCTGGCCGTGAAAAGACA-3′
5′-AACACCGAAGCACCCGATT-3’
59.9
59.9
489 Initiation and maintenance of humoral immune response
BAFF (TNFSF13B) 101,104,901 5’-TTGCAGACAGTGACACGCC-3′
5′-AGGTGTCCCATGGCAAAGG-3’
60.9
59.9
426 B Cell survival
MIF 780,466 5’-GCAAGCCGGCACAGTACAT-3′
5′-ATGTAGATCCTGTCCGGGCT-3’
61.0
60.1
305 Survival of mature B cells + NF-kB signalling cascade
Reference gene
Ovine GAPDH 443,005 5’-AGAAACCTGCCAAGTATGATG-3′
5′-CCTAGAATGCCCTTGAGAGG-3’
60.5
62.6
76