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Table 3 Gene expression changes (fold change) in the ileum of the different treatment groups compared to non-vaccinated control sheep

From: The humoral immune response is essential for successful vaccine protection against paratuberculosis in sheep

 

Non-vaccinated uninfected (n = 10)

Non-vaccinated infected (n = 10)

Vaccinated control (n = 5)

Vaccinated uninfected (n = 18)

Vaccinated infected (n = 2)

 

aIgG1 (SP%)

0.49

10.75

54.39

91.09

52.2

Up regulated

LYN

2.1

1.2

 

1.4

1.6

GRB2

1.8

   

1.4

ERG1

     

JUN

 

1.4

  

1.1

NFIL3

 

2.2

 

1.4

2.8

LCK

1.5

3.2

   

CD81

1.5

1.9

   

CD84

 

3.8 a

   

CD40LG

2.5

5

  

5

BAFF

1.5

5 b

  

2.2

MIF

1.1

2.4

  

1.3

Down regulated

LYN

  

−1.8

  

GRB2

 

−1.2

−3

−1.1

 

ERG1

−1.7

−3.8

−3.3

−1.4

−1.7

JUN

−1.4

 

−1.9

− 1.1

 

NFIL3

−1.1

 

−1.6

  

LCK

  

−2.8

−1

− 1.1

CD81

  

−3.6

−1.6

− 1.2

CD84

−1.1

 

−2.1

−2.4 a

−1.1

CD40LG

  

−1.1

− 1

 

BAFF

  

−1.2

-1 b

 

MIF

  

−2.9

−1.7

 
  1. aIgG1 SP% of each group at the same timepoint as gene expression analysis was performed. Significant differences between groups are indicated by bolding (p < 0.05) with a significantly different to a and b significantly different to b