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Table 3 Primers used for amplification of E. coli and enterotoxins in digesta samples

From: Protective effect of chicken egg yolk immunoglobulins (IgY) against enterotoxigenic Escherichia coli K88 adhesion in weaned piglets

Target gene

Orientation

Sequence (5′-3′)

Tm (°C)

Product size (bp)

Total bacteria

Forward

CGGTCCAGACTCCTACGGG

63

200

Reverse

TTACCGCGGCTGCTGGCAC

  

Escherichia coli

Forward

CCGATACGCTGCCAATCAGT

65

884

Reverse

ACGCAGACCGTAGGCCAGAT

  

K88 fimbriae

Forward

GCACATGCCTGGATGACTGGT

63

439

Reverse

CGTCCGCAGAAGTAACCCCACCT

  

Heat-stable enterotoxin b

Forward

TGCCTATGCATCTACACAAT

63

110

Reverse

CTCCAGCAGTACCATCTCTA

  

Heat-stable enterotoxin a

Forward

ATGAAAAAGCTAATGTTGGC

65

193

Reverse

TACAACAAAGTTCACAGCAG

  

Heat-labile enterotoxin

Forward

CCGTGCTGACTCTAGACCCCCA

68

480

Reverse

CCTGCTAATCTGTAACCATCCTCTGC

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