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Table 2 Blood biochemistry parameters of birds hatching from enrofloxacin- and marbofloxacin-exposed eggs. Values represent mean ± SD; n = 11, 7 and 6 in control, enrofloxacin- and marbofloxacin-exposed birds, respectively; * = p < 0.05, ** = p < 0.01 (when compared against the control group; as there was no significant difference between the control groups, C1 = untreated control and C2 = sham-treated control eggs (aqua pro injectione) were grouped. Blood samples were obtained from chicks on the day of hatching

From: Reproductive toxicity of fluoroquinolones in birds

Parameters

Groups of in ovo exposed birds

Control

Enrofloxacin-treated

Dose E2

Marbofloxacin-treated

Dose M2

TPRO (g/l)

24.00 ± 2.45

17.71 ± 1.70**

20.00 ± 1.55**

BUN (mmol/l)

3.35 ± 1.59

4.46 ± 2.05

3.1 ± 1.14

UA (μmol/l)

429.55 ± 107.33

288.57 ± 139.73*

267.00 ± 75.82*

BUN/UA

7.99 ± 3.77

17.57 ± 8.91*

12.67 ± 5.85

Ca (mmol/l)

2.33 ± 0.41

2.51 ± 0.50

2.52 ± 0.38

IP (mmol/l)

0.74 ± 0.23

0.87 ± 0.16

1.17 ± 0.47*

Mg (mmol/l)

0.75 ± 0.05

0.78 ± 0.08

0.75 ± 0.14

GLU (mmol/l)

11.66 ± 0.59

12.76 ± 0.77*

12.08 ± 1.11

TG (mmol/l)

0.34 ± 0.09

0.61 ± 0.34*

0.61 ± 0.24*

TBIL (μmol/l)

5.09 ± 2.43

6.57 ± 1.40

6.00 ± 1.10

T-Chol (mmol/l)

10.13 ± 0.94

9.50 ± 0.55

9.86 ± 1.07

HDL-Chol (mmol/l)

5.99 ± 0.75

5.66 ± 1.06

6.10 ± 1.24

AMY (μkat/l)

11.98 ± 5.42

9.70 ± 2.28

8.33 ± 4.32

AST (μkat/l)

3.39 ± 1.61

4.17 ± 1.97

4.15 ± 1.15

ALP (μkat/l)

69.10 ± 17.96

66.00 ± 14.93

62.52 ± 28.39

CK (μkat/l)

26.29 ± 16.54

37.95 ± 9.99

29.75 ± 10.60

LDH (μkat/l)

27.42 ± 15.04

34.50 ± 17.46

45.95 ± 19.69

  1. Group labels: control birds (C), data for chicks hatching from untreated and sham-treated control eggs were grouped together in this analysis; birds exposed to fluoroquinolone enrofloxacin (dose E2 = 10 μg of enrofloxacin per 1 g of egg weight) and marbofloxacin (dose M2 = 10 μg of marbofloxacin per 1 g of egg weight)
  2. Abbreviations: TPRO total protein, BUN blood urea nitrogen, UA uric acid, BUN/UA ratio blood urea nitrogen/uric acid, Ca calcium, IP inorganic phosporus, Mg magnesium, GLU glucose, TG triglycerides, TBIL total bilirubin, T-Chol total cholesterol, HDL-Chol high-density lipoprotein cholesterol, AMY amylase, AST aspartate aminotransferase, ALP alkaline phosphatase, CK creatine kinase, LDH lactate dehydrogenase