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Table 3 Primers used to detect BVDV in this study

From: Experimental infection of Korean native goats (Capra aegagrus hircus) with bovine viral diarrhea virus 1b

Target gene

Sequence (5′-3′)

Tm (°C)/Time

Amplicon size

5′-UTR

F, ATG CCC WTA GTA GGA CTA GCA

55 °C, 40 s

288 bp

R,TCA ACT CCA TGT GCC ATG TAC

Npro

F, TCT CTG CTG TAC ATG GCA CAT G

52 °C, 1 min

425 bp

R,CCA TCT ATR CAC ACA TAA ATG TGG T