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Table 1 Antibodies used for flow cytometric analysis of equine cell surface markers

From: The effect of hypoxia on chondrogenesis of equine synovial membrane-derived and bone marrow-derived mesenchymal stem cells

Antibody

Clone/ Isotype

Host Species

Target Species

Fluorophore

2o Antibody

Company

Dilution for 1o Antibody

CD29

TMD29/IgG1

Mouse

Human

APC

Yes

Goat anti-mouse IgG

EMD Millipore

1:100

CD44

IM7/IgG2b

Rat

Human

FITC

No

Thermo IM7

1:80

CD90

?/IgM

Mouse

Canine, Equine

RPE

No

WSU Monoclonal Antibody Center

1:200

CD105

SN6/IgG1

Mouse

Human

Alexa 488

No

Bio Rad

1:10

CD45RB

?/IgM

Mouse

Equine

RPE

No

WSU Monoclonal Antibody Center

1:200

CD79α

HM57/IgG1

Mouse

Human

Alexa 647

No

Bio Rad

1:200

MHCI

cz3/IgG2b

Mouse

Equine

APC

Yes

Goat anti-mouse IgG

Gifta

1:100

MHCII

cz11/IgG1

Mouse

Equine

APC

Yes

Goat anti-mouse IgG

Gifta

1:200

Isotype Control

Corresponding MAB

 

Target Species

Fluorophore

 

Company

Dilution

IgG1

To CD29

 

Mouse

APC

 

Abcam

1:100

IgG2b

To CD44

 

Rat

Alexa 488

 

Abcam

1:100

IgM

To CD90

 

Mouse

PE

 

Abcam

1:200

IgG1

To CD105

 

Mouse

Alexa 488

 

Abcam

1:200

IgM

To CD45RB

 

Mouse

PE

 

Abcam

1:200

IgG1

To CD79α

 

Mouse

Alexa 647

 

Abcam

1:400

IgG2b

To MHCI

 

Mouse

APC

 

Abcam

1:100

IgG1

To MHCII

 

Mouse

APC

 

Abcam

1:100

  1. aGifts from Dr. Doug Antczak, Cornell University, Ithaca, New York, USA