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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Mycobacterium tuberculosis extracellular vesicle-associated lipoprotein LpqH as a potential biomarker to distinguish paratuberculosis infection or vaccination from tuberculosis infection

Fig. 3

Reactivity of bovine sera to M. tuberculosis EV-associated lipoprotein LpqH. a Purified LpqH was separated by SDS-PAGE and submitted to immunoblot with sera from seventeen different cows grouped according to the following treatments: (1) (PTB- TB-), naïve cows; (2) (PTB+) Paratuberculosis positive; (3) (TB+) Bovine tuberculosis positive and; (4) (PTB- Vacc) PTB- and MAP vaccinated cows. Positive reactivity and absence of reactivity are indicated on top of each panel as “+” or “-”, respectively. b Inverse titers of LpqH-specific IgG antibodies measured by ELISA in the same seventeen bovine sera as in (a). Data are mean +/− sem. The results are representative of two independent and similar experiments. (**** P < 0.0001 one-way ANOVA with Tukey post-test)

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