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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Mycobacterium tuberculosis extracellular vesicle-associated lipoprotein LpqH as a potential biomarker to distinguish paratuberculosis infection or vaccination from tuberculosis infection

Fig. 1

EVs from Mycobacterium bovis and Mycobacterium avium paratuberculosis (MAP). a, b Growth curves of M. bovis (a) and MAP (b) measured as OD600 nm. The release of EVs was analyzed by fluorescence measurements after treating clarified supernatants with DPH at the indicated time points. c, d EVs were isolated from cultures of M. bovis (c) and MAP (d) when cultures reached an OD = 0.6 and visualized by cryo-EM. Scale bars: 50 nm. All panels, including the insets represent independent fields of the indicated EV preparations. e, f The size distribution of EVs from M. bovis (e) and MAP (f) were determined by measuring the diameter of EVs in electron micrographs. Data are representative of 2 independent experiments

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BMC Veterinary Research

ISSN: 1746-6148

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