Fig. 4

Immunostaining of donor leucocytes of PP group in the wound bed. a Preparation of PP, PRP, and PRF from DsRed-transgenic mini-pigs. b Immunofluorescence staining. Donor leucocytes in the PP in the wound bed were probed with anti-DsRed antibody and FITC-conjugated secondary antibody (middle). Cell nuclei were visualized with DAPI (left). The merge is shown on the right. The region of FITC-positive cells is circled with a white line. c Immunohistochemistry. The region of donor leucocytes circled with a red line was distinguished using anti-DsRed antibody and peroxidase-conjugated secondary antibody. Scale bar, 50 μm