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Table 6 Performances of SVV real-time RT-PCR (rRT-PCR) and SVV RT-insulated isothermal PCR (RT-iiPCR) on 125 clinical samples using different extraction methods and PCR instruments

From: Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus

  

MagMAXâ„¢/SVV rRT-PCR

Total

Positive

Negative

tacoâ„¢ mini/SVV RT-iiPCR

Positive

71

0

71

Negative

2

52

54

 

Total

73

52

125

  1. Sensitivity: 97.26%; Specificity: 100%; Accuracy: 98.40%
  2. MagMAXâ„¢, MagMAXâ„¢ Pathogen RNA/DNA kits on Kingfisher Flex instrument; tacoâ„¢ mini, tacoâ„¢ Preloaded DNA/RNA Extraction Kit on tacoâ„¢ mini Automatic Nucleic Acid Extraction System instrument
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BMC Veterinary Research

ISSN: 1746-6148

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