BMC Veterinary Research

Table 5 Performances of SVV real-time RT-PCR (rRT-PCR) and SVV RT-insulated isothermal PCR (RT-iiPCR) on 125 clinical samples extracted by the MagMAX method

From: Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus

		SVV rRT-PCR		Total
		Positive	Negative	Total
SVV RT-iiPCR	Positive	73	1	74
SVV RT-iiPCR	Negative	0	51	51
	Total	73	52	125

Sensitivity: 100%; Specificity: 98.08%; Accuracy: 99.20%
Nucleic acid extraction method: MagMAX™ Pathogen RNA/DNA kits on Kingfisher Flex instrument

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ISSN: 1746-6148

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