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Table 1 Primers and probes of SVV real-time RT-PCR and SVV RT-insulated isothermal PCR (RT-iiPCR)

From: Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus

Assay name

Primer & Probe

Nucleotide sequence (5′ - 3′)

Nucleotide Positiona

Target gene

Amplicon

SVV real-time RT-PCR

SVV-F

AACCGGCTGTGTTTGCTAGAG

59–79

5′ UTR

147 bp

SVV-R

GAACTCGCAGACCACACCAA

205–186

SVV-P

6/FAM-CCAAAGGTGTTAGCGCACCCAAACG/IBFQ

143–167

SVV RT-iiPCR

SVV iiF

GAAGCCATGCTCTCCTACTTCAAA

7033–7056

3D

87 bp

SVV iiR

TTCTTTTCCAGAATGTTGAGCCA

7119–7097

SVV iiP

6/FAM-TCGAGAAGCTGCAATCTG/MGB-NFQ

7070–7086

  1. aNucleotide positions of SVV primers and probes are based on GenBank accession no. NC_011349