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Table 6 Relative mRNA levels of MyoD, Myogenin, IFG-1, GH, PAX7 and PCNA in the muscle tissues at days 14 and 28 post hatch in broiler chicks subjected to different thermal manipulation protocols during embryonic life (ED12–18)

From: Thermal manipulation of the broilers embryos: expression of muscle markers genes and weights of body and internal organs during embryonic and post-hatch days

Post-Hatch days

Parameters

Groups

Control

TM1

TM2

TM3

TM4

Day 14

MyoD

1 ± 0.03a

1.16 ± 0.06a

1.53 ± 0.03b

2.29 ± 0.01c

1.64 ± 0.07b

Myogenin

1 ± 0.06a

0.44 ± 0.02b

0.79 ± 0.03c

1.67 ± 0.06d

0.49 ± 0.01b

IGF-1

1 ± 0.04a

1.40 ± 0.04b

1.92 ± 0.05c

2.25 ± 0.02d

1.60 ± 0.09e

GH

1 ± 0.01a

0.39 ± 0.01b

0.56 ± 0.07b

1.40 ± 0.06c

0.51 ± 0.01b

PAX7

1 ± 0.03a

1.09 ± 0.06a

1.34 ± 0.05b

2.02 ± 0.01c

1.29 ± 0.03b

PCNA

1 ± 0.03a

1.28 ± 0.05b

1.63 ± 0.03b

1.56 ± 0.06b

1.73 ± 0.04b

Day 28

MyoD

1 ± 0.03a

1.55 ± 0.03b

3.44 ± 0.11c

1.53 ± 0.02b

1.78 ± 0.01b

Myogenin

1 ± 0.06a

0.10 ± 0.22b

4.44 ± 0.05c

1.04 ± 0.08a

1.30 ± 0.02d

IGF-1

1 ± 0.04a

1.48 ± 0.07b

3.53 ± 0.05c

2.99 ± 0.05d

2.53 ± 0.07e

GH

1 ± 0.01a

1.12 ± 0.01b

4.09 ± 0.07d

1.93 ± 0.05c

1.17 ± 0.06c

PAX7

1 ± 0.03a

1.96 ± 0.06b

3.44 ± 0.13c

1.53 ± 0.06d

2.21 ± 0.13b

PCNA

1 ± 0.03a

1.15 ± 0.02b

1.89 ± 0.16c

1.19 ± 0.03b

1.63 ± 0.05c

  1. TM: Thermal manipulation; MyoD: Myogenic differentiation gene; IGF-1: Insulin-like growth factor 1; GH: Growth hormone; Pax7: Paired box transcription factor; PCNA: Proliferating cell nuclear antigen
  2. a-e Within the same row, means ± SD with different superscripts differ significantly (P < 0.05)