Skip to main content

Table 4 Relative mRNA levels of MyoD, Myogenin, IFG-1, GH, PAX7 and PCNA in the muscle tissues on embryonic days (ED) 12 and 18 in broiler chicks subjected to different thermal manipulation protocols during embryonic life (ED12–18)

From: Thermal manipulation of the broilers embryos: expression of muscle markers genes and weights of body and internal organs during embryonic and post-hatch days

ED Parametrs Groups
Control TM1 TM2 TM3 TM4
ED12 MyoD 1 ± 0.01a 1.11 ± 0.02b 1.14 ± 0.03b 1.02 ± 0.03a 1.63 ± 0.07c
Myogenin 1 ± 0.01a 1.38 ± 0.03b 1.68 ± 0.06c 0.94 ± 0.02d 1.16 ± 0.04e
IGF-1 1 ± 0.01a 0.86 ± 0.02b 0.92 ± 0.03b 1.18 ± 0.01c 1.55 ± 0.08d
GH 1 ± 0.05a 8.90 ± 0.04b 15.8 ± 0.05c 1.47 ± 0.04d 1.03 ± 0.05a
PAX7 1 ± 0.01a 0.85 ± 0.02b 0.92 ± 0.04b 0.79 ± 0.02b 1.38 ± 0.04c
PCNA 1 ± 0.01a 1.07 ± 0.02a 1.11 ± 0.01b 0.99 ± 0.02a 1.19 ± 0.03b
ED18 MyoD 1 ± 0.02a 1.59 ± 0.02b 1.85 ± 0.03b 1.88 ± 0.03b 2.31 ± 0.07c
Myogenin 1 ± 0.11a 2.57 ± 0.53b 4.05 ± 0.16c 2.79 ± 0.12b 21.9 ± 0.44d
IGF-1 1 ± 0.01a 1.29 ± 0.02b 1.72 ± 0.03c 1.37 ± 0.01b 1.85 ± 0.05d
GH 1 ± 0.05a 4.11 ± 0.04b 18.60 ± 0.05c 4.63 ± 0.04b 5.33 ± 0.25d
PAX7 1 ± 0.03a 1.43 ± 0.02b 1.21 ± 0.04c 1.40 ± 0.02b 2.18 ± 0.07d
PCNA 1 ± 0.06a 2.93 ± 0.02b 3.96 ± 0.19c 2.12 ± 0.10b 2.50 ± 0.10b
  1. TM: Thermal manipulation; ED: embryonic day; MyoD: Myogenic differentiation gene; IGF-1: Insulin-like growth factor 1; GH: Growth hormone; Pax7: Paired box transcription factor; PCNA: Proliferating cell nuclear antigen
  2. a-e Within the same row, means ± SD with different superscripts differ significantly (P < 0.05)