BMC Veterinary Research

Table 4 Relative mRNA levels of MyoD, Myogenin, IFG-1, GH, PAX7 and PCNA in the muscle tissues on embryonic days (ED) 12 and 18 in broiler chicks subjected to different thermal manipulation protocols during embryonic life (ED12–18)

From: Thermal manipulation of the broilers embryos: expression of muscle markers genes and weights of body and internal organs during embryonic and post-hatch days

ED	Parametrs	Groups
ED	Parametrs	Control	TM₁	TM₂	TM₃	TM₄
ED12	MyoD	1 ± 0.01^a	1.11 ± 0.02^b	1.14 ± 0.03^b	1.02 ± 0.03^a	1.63 ± 0.07^c
	Myogenin	1 ± 0.01^a	1.38 ± 0.03^b	1.68 ± 0.06^c	0.94 ± 0.02^d	1.16 ± 0.04^e
	IGF-1	1 ± 0.01^a	0.86 ± 0.02^b	0.92 ± 0.03^b	1.18 ± 0.01^c	1.55 ± 0.08^d
	GH	1 ± 0.05^a	8.90 ± 0.04^b	15.8 ± 0.05^c	1.47 ± 0.04^d	1.03 ± 0.05^a
	PAX7	1 ± 0.01^a	0.85 ± 0.02^b	0.92 ± 0.04^b	0.79 ± 0.02^b	1.38 ± 0.04^c
	PCNA	1 ± 0.01^a	1.07 ± 0.02^a	1.11 ± 0.01^b	0.99 ± 0.02^a	1.19 ± 0.03^b
ED18	MyoD	1 ± 0.02^a	1.59 ± 0.02^b	1.85 ± 0.03^b	1.88 ± 0.03^b	2.31 ± 0.07^c
	Myogenin	1 ± 0.11^a	2.57 ± 0.53^b	4.05 ± 0.16^c	2.79 ± 0.12^b	21.9 ± 0.44^d
	IGF-1	1 ± 0.01^a	1.29 ± 0.02^b	1.72 ± 0.03^c	1.37 ± 0.01^b	1.85 ± 0.05^d
	GH	1 ± 0.05^a	4.11 ± 0.04^b	18.60 ± 0.05^c	4.63 ± 0.04^b	5.33 ± 0.25^d
	PAX7	1 ± 0.03^a	1.43 ± 0.02^b	1.21 ± 0.04^c	1.40 ± 0.02^b	2.18 ± 0.07^d
	PCNA	1 ± 0.06^a	2.93 ± 0.02^b	3.96 ± 0.19^c	2.12 ± 0.10^b	2.50 ± 0.10^b

TM: Thermal manipulation; ED: embryonic day; MyoD: Myogenic differentiation gene; IGF-1: Insulin-like growth factor 1; GH: Growth hormone; Pax7: Paired box transcription factor; PCNA: Proliferating cell nuclear antigen
^a-e Within the same row, means ± SD with different superscripts differ significantly (P < 0.05)

Back to article page

ISSN: 1746-6148

Contact us

Submission enquiries: bmcveterinaryresearch@biomedcentral.com
General enquiries: ORSupport@springernature.com