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Table 1 Real-time PCR primers and product length

From: Changes in the blood routine, biochemical indexes and the pro-inflammatory cytokine expressions of peripheral leukocytes in postpartum dairy cows with metritis

Gene (GeneBank ID) PCR primer sequences Product length
β-actin
(NM-173979.3)
F:5′-CATCACCATCGGCAATGAGC-3′
R:5′-AGCACCGTGTTGGCGTAGAG-3’
156 bp
IL-1α
(NM-174092.1)
F:5’CTAAAGGAGATGCCTGAGACACC-3′
R:5’CTGATTTGAAGTAGTCCATAGAGCC-3’
97 bp
IL-1β
(NM-174093.1)
F:5’-TGATGACCCTAAACAGATGAAGAGC-3′
R:5’-CCACGATGACCGACACCACCT-3’
134 bp
IL-6
(EU-276071.1)
F:5’-TGAAAGCAGCAAGGAGACACT-3′
R:5′-TGATTGAACCCAGATTGGAAGC-3’
90 bp
IL-8
(NM-173979.3)
F:5’-TTCCTCAGTAAAGATGCCAATG-3′
R:5′-TGACAACCCTACACCAGACCCA-3’
86 bp
TNF-α
(AF-348421.1)
F:5’-GGGCTTTACCTCATCTACTCACAG-3′
R:5′-GATGGCAGACAGGATGTTGACC-3’
132 bp