Table 1 Real-time PCR primers and product length
Gene (GeneBank ID) | PCR primer sequences | Product length |
|---|---|---|
β-actin (NM-173979.3) | F:5′-CATCACCATCGGCAATGAGC-3′ R:5′-AGCACCGTGTTGGCGTAGAG-3’ | 156 bp |
IL-1α (NM-174092.1) | F:5’CTAAAGGAGATGCCTGAGACACC-3′ R:5’CTGATTTGAAGTAGTCCATAGAGCC-3’ | 97 bp |
IL-1β (NM-174093.1) | F:5’-TGATGACCCTAAACAGATGAAGAGC-3′ R:5’-CCACGATGACCGACACCACCT-3’ | 134 bp |
IL-6 (EU-276071.1) | F:5’-TGAAAGCAGCAAGGAGACACT-3′ R:5′-TGATTGAACCCAGATTGGAAGC-3’ | 90 bp |
IL-8 (NM-173979.3) | F:5’-TTCCTCAGTAAAGATGCCAATG-3′ R:5′-TGACAACCCTACACCAGACCCA-3’ | 86 bp |
TNF-α (AF-348421.1) | F:5’-GGGCTTTACCTCATCTACTCACAG-3′ R:5′-GATGGCAGACAGGATGTTGACC-3’ | 132 bp |