Fig. 2

Growth kinetics of DHAV-3 in DEF cells. DEF cells were inoculated with the 5th cell passages of DHAV-3 YDF120 and C-GY at a MOI corresponding to 1 copy/cell. Viral yields in DEF cells were reflected by RNA copies detected with qPCR at indicated time after inoculation. At each time point, samples (medium together with cells) (a) and medium (b) were collected from three wells of 24-well-plates. Error bars represent the SD (n = 3). *, P < 0.05; **, P < 0.01