TY - JOUR AU - Wang, Minghang AU - Li, Ziheng AU - Liu, Huicong AU - Wang, Xiaoyan AU - Zhang, Dabing PY - 2019 DA - 2019/05/17 TI - Effect of fetal calf serum on propagation of duck hepatitis A virus genotype 3 in duck embryo fibroblast cells JO - BMC Veterinary Research SP - 153 VL - 15 IS - 1 AB - Duck viral hepatitis (DVH) is a highly contagious viral disease affecting ducks. It can be caused by five agents, including duck hepatitis A virus genotypes 1 (DHAV-1), 2 (DHAV-2), and 3 (DHAV-3), as well as duck hepatitis virus 2 and duck hepatitis virus 3. Since 2007, DHAV-3 has been known to be the most prevalent in East and South Asia. So far, the information regarding the propagation of DHAV-3 in cultured cells is limited. In this study, we describe the comparative studies on the growth properties of DHAV-3 in primary duck embryo fibroblast (DEF) cells using two different strains: a virulent strain C-GY and an attenuated strain YDF120. The effect of fetal calf serum (FCS) and chick serum (CS) on DHAV-3 replication and the mechanism of the inhibitory effect conferred by FCS were also investigated. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-019-1904-y DO - 10.1186/s12917-019-1904-y ID - Wang2019 ER -